Archives of Biological Sciences 2011 Volume 63, Issue 2, Pages: 371-380
https://doi.org/10.2298/ABS1102371L
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Genetic divergence of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by RAPD
Lučić A. (Institute of Forestry, Belgrade)
Isajev V. (Faculty of Forestry, Belgrade)
Rakonjac L. (Institute of Forestry, Belgrade)
Ristić Danijela 
(Maize Research Institute, Zemun Polje)
Kostadinović Marija (Maize Research Institute, Zemun Polje)
Babić Vojka (Maize Research Institute, Zemun Polje)
Nikolić Ana (Maize Research Institute, Zemun Polje)
The ability of random amplified polymorphic DNA (RAPD) to distinguish among
Scots pine populations from Serbia was evaluated. Sixteen arbitrary 10-mer
primers employed in the analysis produced 54 fragments of which 21 were
polymorphic (38.89%). Certain rare and genotype-specific bands were
identified which could be effectively used to distinguish between the
populations. Polymorphism in RAPD markers among P. sylvestris populations was
high and sufficient to distinguish each of the populations. The results
obtained suggest that RAPD markers are valuable for the genetic divergence
estimation in Pinus sylvestris and for the study of divergence among
populations.
Keywords: Pinus sylvestris, population, RAPD, polymorphism