doiSerbia

Effects of anoxia on 31P NMR spectra of Phycomyces blakesleeanus during development

Stanić Marina (Biološki fakultet, Beograd)
Živić M. (Institut za opštu i fizičku hemiju, Beograd)
Zakrzewska Joana (Institut za opštu i fizičku hemiju, Beograd)

The method of 31P NMR spectroscopy was used to investigate the effects of anoxia on Phycomyces blakesleea­nus mycelium during development. The greatest changes were recorded in the PPc, NADH, and α-ATP signals. Decrease of PPc signal intensity is due to chain length reduction and reduction in number of PPn molecules. Smaller decrease of β-ATP compared to α-ATP signal intensity can be attributed to maintenance of ATP concentration at the expense of PPn hydrolysis. Sensitivity to anoxia varies with the growth stage. It is greatest in 32-h and 44-h mycelium, in which PPn is used as an additional energy source, while the smallest effect was noted for 36-h fungi.

Keywords: 31P NMR, anoxia, Phycomyces blakesleeanus, development, polyphosphates